产品货号:
Z10824
中文名称:
14-3-3 Sigma/SFN抗体
英文名称:
Anti-14-3-3 Sigma/SFN Antibody
产品规格:
50μl|100μl|150μl
发货周期:
1~3天
产品价格:
询价
抗体名称 | Anti-14-3-3 Sigma/SFN Antibody |
指标别名 | 14 3 3 protein sigma;14-3-3;14-3-3 sigma;HME1;SFN;stratifin;YWHAS |
克隆性 | Polyclonal |
检验物种 | human,mouse,rat |
应用范围 | WB,IHC,IF,FCM,ICC,IHC-F,ELISA |
基因名称 | SFN |
抗体来源 | Rabbit |
抗体类型 | IgG |
免疫原 | E.coli-derived human 14-3-3 sigma recombinant protein (Position:M1-S248). |
实际分子量 | 28KD |
成分 | 500μg/mL antibody with PBS,0.02% NaN3,1mg BSA and 50% glycerol. |
纯化方式 | Immunogen affinity purified. |
浓度 | 500μg/mL |
产品形态 | 溶液 |
保存条件 | 12 months from date of receipt,-20℃ as supplied.6 months 2 to 8℃ after reconstitution.Avoid repeated freezing and thawing. |
背景资料 | Stratifin(SFN),also known as 14-3-3 protein sigma,is strongly induced by gamma irradiation and other DNA-damaging agents.The induction of 14-3-3-sigma is mediated by a p53 -responsive element located 1.8kb upstream of its transcription start site.The protein,called stratifin,was shown to be diffusely distributed in the cytoplasm and was present in cultured epithelial cells.It was most abundant in tissues enriched in stratified keratinizing epithelium. |
Uniprot ID | P31947 |
推荐配套试剂 | Biorab recommends Enhanced Chemiluminescent Kit with anti-Rabbit IgG (ZN1926) for Western blot,and HRP Conjugated anti-Rabbit IgG Super Vision Assay Kit (ZN1865) for IHC(P).*Blocking peptide可以联系我们购买。 |
推荐稀释比 | Western blot (WB) 1:500~2000 Immunohistochemistry in paraffin section (IHC) 1:50~400 Immunohistochemistry in frozen section 1:50~400 Immunocytochemistry in fixed cells 1:50~400 Flow cytometry (FCM) 1~3μg/1×106 cells ELISA: 1:100~1000 (Boiling the paraffin sections in 10mM citrate buffer,pH6.0,or PH8.0 EDTA repair liquid for 20 mins is required for the staining of formalin/paraffin sections.) Optimal working dilutions must be determined by end user. |
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